节点文献

重组极耐热内切葡聚糖酶Cel12B的诱导条件及酶的纯化

Inducement condition and purification of recombinated thermostable endoglucanase Cel12B expressed in E coli

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 LI Xiang-qian1,2, SHAO Wei-lan1,3(1.The Key Laboratory of Industrial Biotechnology under Ministry of Education, Southern Yangtze University, Wuxi 214036; 2.Huaiyin Institute of Technology, Huai’an 223001; 3.School of Life Science, Nanjing Normal University, Nanjing 210097)

【机构】江南大学工业生物技术教育部重点实验室；江南大学工业生物技术教育部重点实验室无锡214036淮阴工学院；淮安223001；无锡214036南京师范大学生命科学院；南京210097；

【摘要】构建重组菌(JM109/pET-20b-Cel12B)生产极耐热内切葡聚糖酶,研究了不同表达宿主、IPTG诱导时间、IPTG浓度、重组菌生长不同阶段添加IPTG对重组菌生产极耐热内切葡聚糖酶的影响。结果表明:最佳诱导时间是OD值为1.0,IPTG浓度从0.5到3.0对酶活表达影响不大,诱导8h后极耐热内切葡聚糖酶表达量基本不变,优化后重组菌(E.coliBL21-CodonPlus(DE3)-RIL/pET-20b-Cel12B)表达酶活比原来菌株(E.coliJM109(DE3)/pET-20b-Cel12B)提高了53.9%,经过热处理和亲和层析得到电泳纯的蛋白条带。更多还原

【Abstract】 Several factors influencing thermostable endoglucanase production with recombination E coli(JM109/pET-20b-Cel12B)were investigated.The results showed that the production of thermostable endoglucanase did not improve after 8 hours of IPTG induction.Concentration of IPTG from 0.5 to 3mmol/L had almost the same effect on erpression of thermostable endoglucanase.The best induced time was OD600 at about 1.0. Enzyme activity produced by E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-Cel12B was 1.539 times higher than that by E.coli JM109(DE3)/ pET-20b-Cel12B. The recombinant protein was purified by heating and immobilizing metal affinity chromatography.更多还原

【关键词】重组菌；诱导；内切葡聚糖酶；
【Key words】 re-combination E coli； induction； endoglucanase；

【基金】江苏省教育厅自然科学研究项目(05KJB180006)

【文献出处】食品科技 ,Food Science and Technology , 编辑部邮箱 ,2006年10期

【分类号】TS201.2
【下载频次】127

知网节下载

节点文献中：

本文链接的文献网络图示:

本文的引文网络

节点文献