【摘要】 为建立一种同时检测食品中的伤寒沙门氏菌(ST)、金黄色葡萄球菌(SA)和单增李斯特氏菌(LM)的快速检测方法,分别针对伤寒沙门氏菌的鞭毛抗原基因H1-d、金黄色葡萄球菌的耐热核酸酶基因nuc、单增李斯特氏菌溶血素O上的hlyA基因设计引物,进行特异性和灵敏性实验,结果表明3条特异性扩增片段分别为458bp、279bp和243bp,经DNA测序证明其序列与模板被扩增片段一致。该方法操作简便、快速,具有良好的灵敏性和特异性。更多还原

【Abstract】 For establish a rapid method to test sallmonella typhi, staphylococcus aureus and listeria monocytogenes in food, so designed three specific primers according to ST, SA and LM. The target gene was amplified from the three bacterium chromosomal DNA by using PCR technique. Then we detect that if the method was specific and sensitive. Electrophoresis products indicate there are three strips in 458bp, 279bp and 243bp. Extraction the target strips sequence analysis indicate nucleotide sequences are consistent with the templates. Proving the specimen are positive. The products reveal that this method provides a specific, sensitive, swift and simple means for the three bacterium mentioned above.更多还原