【摘要】 建立了中空纤维膜液相微萃取-高效液相色谱(HPLC)测定醋酸氯己定痔疮栓中醋酸氯己定含量的方法。将样品用醋酸水溶液萃取5次,配成样品溶液,然后用装有正辛醇的中空纤维膜进行液相微萃取,萃取液用高效液相色谱测定。醋酸氯己定的质量浓度为0.5~16 mg/L时与其峰面积呈良好的线性关系,加样回收率大于98.0%,相对标准偏差低于4.0%。该方法样品处理简单、快速,灵敏度与不经过液相微萃取的HPLC方法相比提高了约24倍,醋酸氯己定痔疮栓中的其他物质对测定无干扰。更多还原

【Abstract】 A novel method was developed to determine the content of chlorhexidine acetate(CA) in chlorhexidine acetate suppositories by high performance liquid chromatography(HPLC) coupled with hollow fiber membrane solvent microextraction.The sample was extracted with 1.5 mol/L acetic acid solution five times,then a hollow fiber containing n-octanol was placed in the sample solution to perform microextraction for extracting 20 min.The extract was analyzed by HPLC with UV detection at 260 nm.The chromatographic conditions were as following: acetonitrile-redistilled water(29∶71,v/v,containing 0.3% triethylamine,pH 3.0) as mobile phase with the flow rate of 1 mL/min.The calibration curve of CA was linear from 0.5 to 16 mg/L.The method recovery was over 98.0% with relative standard deviation less than 4.0%.By using hollow fiber membrane solvent microextraction,the enrichment of 24-fold was achieved.The method is specific,simple,fast,sensitive and suitable for the determination of chlorhexidine acetate.更多还原