【摘要】 对蒙古黄芪中5种异黄酮类成分的含量进行了反相高效液相色谱法测定。色谱柱为D iam ons il C18柱,流动相为乙腈-水系统,梯度洗脱,检测波长230nm,柱温35℃。毛蕊异黄酮-7-O-β-D-葡萄糖苷在20.12~201.2m g/L、芒丙花素-7-O-β-D-葡萄糖苷在4.62~46.2m g/L、9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷在4.86~48.6m g/L、毛蕊异黄酮在9.24~92.4m g/L、芒丙花素在6.92~69.2m g/L时峰面积与浓度呈良好的线性关系,相关系数分别为0.999 2,0.999 7,0.999 7,0.999 5和0.999 5。5种成分的加样回收率均高于94%,相对标准偏差(RSD)小于3.2%(n=9)。该法简便快速,重复性良好,结果准确可靠,可用于黄芪药材中5种主要异黄酮类成分的含量测定。更多还原

【Abstract】 A reversed-phase high performance liquid chromatographic method（RP-HPLC） was developed for the simultaneous analysis of five isoflavonoids of Huangqi,the roots of Astragalus mongholicus.HPLC determination was performed on a Diamonsil C₁8 column（200 mm × 4.6 mm i.d.,5 μm） and detected at 230 nm.The mobile phase consisted of（A）acetonitrile and（B）water.Gradient program was adopted as follows: 0-25 min from 17%A to 31%A,25-45 min 35%A.The flow rate was maintained at 1.0 mL/min.The method was proved to be linear in the ranges of 20.12-201.2 mg/L（r=0.9992）,4.62-46.2 mg/L（r=0.9997）,4.86-48.6 mg/L（r=0.9997）,9.24-92.4 mg/L（r=0.9995） and 6.92-69.2 mg/L（r=0.9995）for the five isoflavonoids,calycosin-7-O-β-D-glucoside,formononetin-7-O-β-D-glucoside,9,10-dimethoxypterocarpan-3-O-β-D-glucoside,calycosin and formononetin,respectively.The recoveries were greater than 94%,and relative standard deviations were less than 3.2%.The method has been successfully applied to the simultaneous determination of the five isoflavonoids of radix astragali.更多还原