【摘要】 建立了蛋黄磷脂中卵磷脂(即磷脂酰胆碱,PC)的高效液相色谱-蒸发光散射检测(HPLC-ELSD)的测定方法。以N ova-Pak S ilica60A硅胶柱(3.9mm i.d.×150mm,4μm)为分离柱,正己烷-异丙醇-3%冰醋酸水溶液(体积比为35∶65∶8)为流动相,等度洗脱,流速1.0m L/m in,柱温30℃。蒸发光散射检测器漂移管温度50℃,雾化气(空气)压力350kPa。在上述条件下测得PC在0.16~1.61g/L范围内线性关系良好(r2=0.997 9),检测限为0.64μg,方法的精密度为3.2%(n=5),回收率为98.2%~128.2%。将该方法用于实际样品的测定,获得了令人满意的结果。该方法预处理简单,分析速度快,可用于蛋黄磷脂中卵磷脂的测定。更多还原

【Abstract】 A method for the determination of egg-yolk phosphatidylcholine by normal-phase high performance liquid chromatography(NP-HPLC) with evaporative light scattering detection(ELSD) was developed.Yolk phosphatidylcholine was successfully separated on a Nova-Pak Silica 60A column(3.9 mm i.d.×150 mm,4 μm),using hexane-isopropanol-water containing 3% acetic acid(35∶65∶8,v/v/v) as mobile phase at a flow rate of 1.0 mL/min.The drift tube temperature and air carrier gas pressure of the ELSD were set at 50 ℃ and 350 kPa,respectively.The linear detection range was 0.16-1.61 g/L(r~2=0.9979) and the limit of detection(LOD) was 0.64 μg with excellent relative standard deviation(RSD) of 3.2%(n=5).The recovery was in the range of 98.2%-128.2%.The results indicated that this method is accurate,rapid,simple and reproducible,and thus suitable for the analysis of egg-yolk phosphatidylcholine in real samples.更多还原