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毛细管电泳法手性拆分14种二肽
Chiral Separation of Dipeptides by Capillary Electrophoresis
【摘要】 以咔唑-9-乙基氯甲酸酯(CEO C)作为柱前衍生试剂,采用毛细管电泳对14种二肽进行了手性拆分。以5种二肽为代表,考察了缓冲液种类、浓度、pH值、二元手性选择剂的组合配比等因素对二肽的拆分效果,优化了实验条件。在各自的优化条件下,14种二肽手性拆分的分离度均在3.63以上,最高分离度可达43.14(G ly-A la)。
【Abstract】 Peptides are increasingly used as pharmaceutical agents.Many small peptides are the essential compounds in biological systems.Direct chiral separation of dipeptide derivatives using 9-(2-carbazole) ethyl chloroformate(CEOC) as the derivatizing agent by capillary(electrophoresis)(CE) with β-cyclodextrin(β-CD) and sodium deoxycholate(SDC) as chiral(selectors) has been developed.It has been well recognized that the combination of the binary(selectors) can enhance the selectivity and resolution instead of either β-CD or SDC alone.The(molar) ratio of the binary chiral selectors,the buffer concentration and pH of Tris-H3PO4,organic modifier were studied and optimized.Complete chiral separations for 14 dipeptide derivatives(using) β-CD and SDC as binary chiral selectors were obtained.The results indicated that each pair of D/L chiral resolution was more than 3.63,and the highest resolution for Gly-Ala was 43.14.
【Key words】 capillary electrophoresis(CE); derivatizing agent; chiral selectors; chiral separation; dipeptide;
- 【文献出处】 色谱 ,Chinese Journal of Chromatography , 编辑部邮箱 ,2006年02期
- 【分类号】O658.9;O629.72
- 【被引频次】3
- 【下载频次】242