【摘要】 以咔唑-9-乙基氯甲酸酯(CEO C)作为柱前衍生试剂,采用毛细管电泳对14种二肽进行了手性拆分。以5种二肽为代表,考察了缓冲液种类、浓度、pH值、二元手性选择剂的组合配比等因素对二肽的拆分效果,优化了实验条件。在各自的优化条件下,14种二肽手性拆分的分离度均在3.63以上,最高分离度可达43.14(G ly-A la)。更多还原

【Abstract】 Peptides are increasingly used as pharmaceutical agents.Many small peptides are the essential compounds in biological systems.Direct chiral separation of dipeptide derivatives using 9-（2-carbazole） ethyl chloroformate（CEOC） as the derivatizing agent by capillary（electrophoresis）（CE） with β-cyclodextrin（β-CD） and sodium deoxycholate（SDC） as chiral（selectors） has been developed.It has been well recognized that the combination of the binary（selectors） can enhance the selectivity and resolution instead of either β-CD or SDC alone.The（molar） ratio of the binary chiral selectors,the buffer concentration and pH of Tris-H₃PO₄,organic modifier were studied and optimized.Complete chiral separations for 14 dipeptide derivatives（using） β-CD and SDC as binary chiral selectors were obtained.The results indicated that each pair of D/L chiral resolution was more than 3.63,and the highest resolution for Gly-Ala was 43.14.更多还原