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红毛藻藻红蛋白的分离纯化及性质研究
Purification and Characterization of Phycoerythrin from Bangia fusco-purpurea
【摘要】 从红毛藻(Bangiafusco-purpurea)中分离纯化得到纯度较高(A565/A280为5.0)的藻红蛋白(phycoerythrin,PE)。纯化过程包括:硫酸铵分级沉淀,阴离子交换柱层析,凝胶过滤柱层析。通过扫描藻红蛋白的紫外可见光谱,确定其为R-藻红蛋白。用SDS-PAGE及银染色法测定了组成R-藻红蛋白各亚基的相对分子量。并研究了pH值、温度、光照等理化因子对R-藻红蛋白稳定性的影响。
【Abstract】 Phycoerythrin was isolated and purified from Bangia fusco-purpurea. After the successive procedures of ammonium sulfate fraction precipitation, ion-exchange column chromatography, and gel filtration chromatography, the purity (A565/A280) was 5.0. Absorption spectrum characteristic of the purified PE showed that it is R-PE. Relative molecular weight of subunits consisting of R-PE was detected by SDS-PAGE followed by silver staining. The stability of R-PE was investigated under a broad range of pH, different temperatures and light exposure.
【Key words】 Bangia fusco-purpurea; phycoerythrin; purification; stability;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2006年12期
- 【分类号】TS201.21
- 【被引频次】14
- 【下载频次】427