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红毛藻藻红蛋白的分离纯化及性质研究

Purification and Characterization of Phycoerythrin from Bangia fusco-purpurea

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【作者】 付晓苹周琼翁凌苏文金曹敏杰

【Author】 FU Xiao-ping,ZHOU Qiong,WENG Ling,SU Wen-jin,CAO Min-jie* (School of Bioengineering, Jimei University, Xiamen 361021, China)

【机构】 集美大学生物工程学院集美大学生物工程学院 福建厦门361021福建厦门361021

【摘要】 从红毛藻(Bangiafusco-purpurea)中分离纯化得到纯度较高(A565/A280为5.0)的藻红蛋白(phycoerythrin,PE)。纯化过程包括:硫酸铵分级沉淀,阴离子交换柱层析,凝胶过滤柱层析。通过扫描藻红蛋白的紫外可见光谱,确定其为R-藻红蛋白。用SDS-PAGE及银染色法测定了组成R-藻红蛋白各亚基的相对分子量。并研究了pH值、温度、光照等理化因子对R-藻红蛋白稳定性的影响。

【Abstract】 Phycoerythrin was isolated and purified from Bangia fusco-purpurea. After the successive procedures of ammonium sulfate fraction precipitation, ion-exchange column chromatography, and gel filtration chromatography, the purity (A565/A280) was 5.0. Absorption spectrum characteristic of the purified PE showed that it is R-PE. Relative molecular weight of subunits consisting of R-PE was detected by SDS-PAGE followed by silver staining. The stability of R-PE was investigated under a broad range of pH, different temperatures and light exposure.

【关键词】 红毛藻藻红蛋白分离纯化稳定性
【Key words】 Bangia fusco-purpureaphycoerythrinpurificationstability
  • 【分类号】TS201.21
  • 【被引频次】14
  • 【下载频次】427
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