【摘要】 通过正交试验和单因素试验确定了本实验室乳酸克鲁维酵母菌株产乳糖酶的发酵培养基组分(W/V,%)为乳糖8、葡萄糖0.5、酵母膏0.7、尿素0.15、KH2PO41.5、MgSO40.1、MnCl20.01,并考察了其发酵工艺条件,优化得到的最高产酶量平均达到1.930ONPGU/ml。更多还原

【Abstract】 The fermentation culture medium of lactase-producing yeast Kluyveromyces lactis was optimized by orthogonal design and single-factor experiments. The optimal culture medium for fermentation of lactase-producing yeast Kluyveromyces lactis was shown as follows (%, W/V): lactose 8, glucose 0.5, yeast extract 0.7, urea 0.15, KH2PO4 1.5, MgSO4 0.1, MnCl2 0.01. The conditions of fermentation were also investigated in the trail and the average productivity of lactase was 1.930 ONPG unitsp erm illiliter.更多还原