【摘要】 采用反相高效液相色谱法,一次进样,同时测定食品中常见的9种食品添加剂:苯甲酸、山梨酸、糖精钠、安赛密、柠檬黄、日落黄、胭脂红、苋菜红、咖啡因。实验采用Nova-PakC18柱(3.9mm×150mm,4μm,Waters)为分离柱,以20mmol/L乙酸铵(pH6.8)-甲醇为流动相(梯度洗脱:甲醇:5%5min;5%～30%,12.5%/min;30%继续3min;30%～5%,25%/min),采用紫外检测器在234nm下进行检测。整个分析过程在10min内完成。其平均加标回收率为77.0%～108.5%,相对标准偏差小于5%。该方法结果准确,适用于食品常规质量检测。更多还原

【Abstract】 A novel method is proposed for the simultaneous separation and determination of night food additives,saccharin sodium,acesulfame potassium,benzoic acid,sorbic acid,carmine,amaranth,tartrazine,sunset yellow and caffeine by reversed- phase high performance liquid chromatography. The separation was achieved within 10 min by using a Nova-Pak reverse phase C18 column (150mm×3.9mm,4μm,Waters Inc) with 20mmol/L NH4Ac (pH6.8)-methanol as mobile phase (gradient elution: methanol: 5% 5min; 5%～30%,12.5%/min; 30% continued 3min; 30%～5%,25%/min),and lastly detected at 234nm through a UV detector. The average recoveries for real samples ranged from 77.0% to 108.5%.更多还原