【摘要】 本文建立了高效液相色谱法快速测定长鞭红景天中红景天苷和酪醇含量的方法。色谱柱AgilentRP-C18(3.9×50mm,3.5μm);流动相为水-甲醇(80:20);流速为1ml/min;检测波长276nm。红景天苷和酪醇在1.5～30μg和0.8～16μg范围内线性关系良好(r=0.9999),平均回收率分别为98.5%和98.2%。实验3min即可完成测定,方法简便,快速,结果可靠,可用于优良单株的快速筛选。更多还原

【Abstract】 This paper established an RP-HPLC method to determine the contents of salidroside and tyrosol in Rhodiola fastigiat. The separation was performed on a C18( 3.9×50mm,3.5μm),water-methanol(80:20),the mobile phase as at a flow rate of 1ml/min. The detection wavelength was 276nm. Linear ranges were between 1.5～30μg and 0.8～16μg respectively,r=0.9999. The average recoveries were 98.5% and 98.2% respectively. The analysis was finished within 3 minutes. This method is sensitive,quick,simple and accurate for the simultaneous measurement of salidroside and tyrosol in Rhodida. It can be used to select the quality species quickly.更多还原