【摘要】 杜仲愈伤组织生长过于致密从而导致难以实现悬浮培养,因此获得疏松、生长快速愈伤组织是实现杜仲细胞悬浮培养的前提。本文研究了不同外植体、激素和培养基对杜仲愈伤组织诱导率、疏松程度以及褐化率的影响,结果表明:上胚轴和子叶更容易被诱导为疏松愈伤组织,MS培养基是最适诱导培养基;适当增加6-BA浓度有助于褐化的减轻且提高愈伤组织疏松程度,杜仲疏松愈伤组织诱导最适激素配比为1.0mg/L2,4-D+1.0mg/LNAA+1.0mg/L6-BA。更多还原

【Abstract】 Callus from Eucommia ulmoides grows too rigidly to suspension culture,so rapidly vegetal and looses callus is the basis of it’s cell suspension culture. The sterile seedling as explants,the effects of different explants,concentration of NAA,2,4-D and 6-BA and 3 basic medium on Eucommia ulmoides callus induction and growth were investigated. The results showed that the epicotyl and cotyledon are much easier and the MS medium is the best medium for callus induction. The results of L9(34) orthogonal test indicated that properly increasing 6-BA content could reduce darkening and improve loose callus formation,and the optimum hormone combination was 1.0mg/L2,4-D+1.0mg/LNAA+1.0mg/6-BA.更多还原