【摘要】 在装备了PDA检测器的HPLC上,应用C30柱,姜黄乙醇萃取物中的类姜黄素(curcuminoids)组分获得了良好的分离。分离条件为:固定相为YMCTMCarotenoidS-5(4.6×250mm)柱;流动相A为乙腈:水(80:20,V/V),用磷酸调至pH2.5;流动相B为乙腈:水(95:5,V/V),用磷酸调至pH2.5;线性梯度:B在15min内由0%增至100%;流速=1.0ml/min;PDA波长范围为300～550nm;检测波长为430nm;进样量为20μl。根据各组分的色谱行为和光谱特征分析,3种类姜黄素同系物——姜黄素(curcumin)、去甲氧基姜黄素(demethoxycurcumin)及去二甲氧基姜黄素(Bisdemethoxycurcumin)被鉴定。本研究的结果奠定了应用C30-HPLC-PDA定量检测类姜黄素同系物的基础。更多还原

【Abstract】 Curcuminoids from the alcohol extract of Curcuma Longa L root were readily separated by HPLC equipped with a PDA detector on a C30 column under following conditionStationary phase=YMCTM Carotenoid S-5 column (250×4.6mm); Mobile phase A=acetonitrile-water (80:20,V/V) with pH 2.5 adjusted by phosphate; Mobile phase B=acetonitrile:water (95: 5,V/V) with pH 2.5 adjusted by phosphate; Linear gradient: B increased from 0 to 100% (V/V) in 15 minutes; Flow rate=1.0ml/ min. PDA wavelength range: 300～550nm; Sample injection volume=20μl. According to their chromatographic behavior and spectral characters,three homologues,curcumin,demethoxy curcumin and bisdemethoxy curcumin,were identified. Results of this study would form a base to quantify curcuminoid homologues by C30-HPLC-PDA.更多还原