【摘要】 通过增殖抑制试验、细胞形态观察和细胞周期测定,研究了同工麦胚凝集素II(WGAII)对于体外培养的MCF-7和SHZ两种乳腺癌细胞的增殖抑制作用。结果表明最大抑制率均出现于加药后72h,其中SHZ尤为敏感。WGAII对SHZ细胞的LD50为10μg/ml。WGAII作用使细胞皱缩或拉长,部分细胞簇集成团,有些细胞脱壁,细胞表面反光性变差。细胞膜皱缩、外突或粘连,有的小泡破裂,或脱落形成凋亡小体。WGAII将SHZ细胞阻断在G1/S期,干扰了肿瘤细胞的正常增殖活动。更多还原

【Abstract】 In vitro growth inhibition tests, cell shape observations, and cell cycle determinations were conducted to evaluate the inhibition effects of wheat germ agglutinin isolectin II (WGAII) on two breast cancer cell lines, MCF-7 and SHZ. WGA demonstrated inhibitive effects on the proliferation of both cell lines showing maximum inhibition at 72h after incubation. SHZ was more sensitive to WGAII treatment, its LD50 being ￣10μg/ml. WGAII treatment crimpled, stretched, clustered, and detached the cancer cells, and worsens their surface reflectivity. The wall of some cells was blebbed, conglutinated, with some of the blebs broken or detached as apoptotic bodies. WGAII blocked the SHZ cell cycle at the G1/S phase, and thus interfered with the normal proliferation of cancer cells.更多还原