【摘要】 本文研究了油菜花粉中的蛋白质组分分布以及碱浓度、pH值、浸提时间和温度对油菜花粉中碱溶谷蛋白提取率的影响,并在此基础上确定了谷蛋白的最佳提取条件。研究结果表明,破壁脱脂后的油菜花粉中蛋白质组分分布为清蛋白34.66%,球蛋白1.930%,醇溶蛋白2.810%,碱溶谷蛋白49.56%,复合蛋白11.04%,其中主要是清蛋白和谷蛋白;谷蛋白的最佳提取条件是料液比为1:20,氢氧化钠浓度0.30%,pH值为11,浸提时间75min,温度为70℃。更多还原

【Abstract】 Study was conducted on the effect of protein composition distribution in rape pollen, alkali concentration, pH value, time and temperature on the extraction percent of glutelins in rape pollen. The protein composition distribution of defatted and wall-broken rape pollen is albumin 34.66%, globulin 1.930%, prolamine 2.810%, glutelins 45.96%, complex protein 11.04%, and the major protein in rape pollen were albumin and glutelins. Through orthogonal test,the optimum conditions of extraction were obtained on the basis of single factor test, as follow: ratio of solid-liquid1:20, NaOH concentration 0.30%, pH value 11, time 75min, temperature 70℃.更多还原