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菜籽饼粕多糖的超声辅助碱提、纯化及理化性质研究

Study on Extraction by Ultrasonic-NaOH, Purification and Physicalcochemical Characteristic Assay of Polysaccharide on Repseed Meal

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【Author】 CHEN Hao1,FENG Rui1,SHEN Min1,XUE Ai-fang1,WU Mou-cheng2 (1.College of Science, Huazhong Agricultural University, Wuhan 430070, China; 2.College of Food Science and Technology , Huazhong Agricultural University, Wuhan 430070, China)

【机构】华中农业大学理学院；华中农业大学食品科学技术学院湖北武汉430070；湖北武汉430070；

【摘要】本文采用超声辅助氢氧化钠提取菜籽饼粕中的多糖(RSPS),优化了提取条件,并对其进行Sevage法脱蛋白,DEAE-SephadexA-25柱和SephadexG-100柱分离,得到四个级分多糖(RSPS1～4),进而初步研究了其理化性质,并对其中两个较纯级分的多糖采用HPLC法进行了单糖组成分析。更多还原

【Abstract】 The polysaccharide on repseed meal was extracted with ultrasonic-NaOH in this paper, the condition was optimized, and the protein was removed by Sevage, RSPS1～4 were obtained by purify RSPS by DEAE-Sephadex A-25 and Sephadex G- 100. The physical and chemical characteristic of RSPS1～4 were analyzed and the monosaccharide content of RSPS-2 and RSPS-3 were analyzed by HPLC.更多还原

【关键词】超声辅助碱提；菜籽多糖；分离纯化；理化性质分析；单糖组成；
【Key words】 extraction with ultrasonic-NaOH； polysaccharide on rapseed meal； isolation and purification； physicalcochemical characteristic assay； monosaccharide component；

【文献出处】食品科学 ,Food Science , 编辑部邮箱 ,2006年10期

【分类号】TQ28
【被引频次】9
【下载频次】482

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