【摘要】 微生物谷氨酰胺转胺酶(MTG)可通过催化蛋白质谷氨酰胺的酰基转移反应改变蛋白质的结构和性质,是一种有广泛应用前景的重要新型酶制剂。本文根据微生物谷胺酰胺转胺酶的基本理化性质,先后采用了高速离心、硫酸铵盐析沉淀、乙醇沉淀、丙酮沉淀等方法,对微生物谷胺酰胺转胺酶的初步提纯工艺进行了研究讨论,确定了该酶不同用途的初提方法,并为该酶的进一步纯化做好准备。更多还原

【Abstract】 The enzyme of microbial transglutaminase (MTG) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a γ-carboxyamide group of glutamine residue in a peptide chain, resulting the cross-linking of proteins. In this paper, based on the general physical and chemical character of MTG, several satisfied methods was decided after studied on using high centrifugation, ammonium sulfate precipitation, alcohol precipitation, acetone precipitation to separation MTG.更多还原