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微波法提取虫草素研究

Study on Extraction of Cordycepin with Microwave

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【作者】 夏敏温鲁

【Author】 XIA Min1,WEN Lu2 (1.Instrumental Analysis Center, Department of Chemistry, Huaiyin Teachers College, Huaian 223300, China; 2.Department of Biology, Huaiyin Teachers College, Huaian 223300, China)

【机构】 淮阴师范学院化学系分析测试中心淮阴师范学院生物系 江苏淮安223300江苏淮安223300

【摘要】 对蛹虫草固体培养物中的虫草素进行微波提取,用HPLC法测定提取液中的虫草素含量,通过正交试验,确定了微波提取虫草素的最佳条件为:提取液用量10ml,固液比1:200,中火处理3min,样品中虫草素的测定值达12.16mg/g。本法提取时间仅为超声波提取的1/40,且提取率高于超声波提取。

【Abstract】 The cordycepin in culture medium of the cordyceps militaris link were extracted by microwave treatment. HPLC was used to determine the content of cordycepin in extracting solution of cordyceps militaris link. And the orthogonal test design L9 (34) was used to optimize the conditions of microwave treatment. The optimum conditions of microwave treatment were as follows: extracting solution volume 10ml, solid-liquid ratio 1:200, middle firepower treating (microwave irradiation power 50W) for 3min. Content of the cordycepin in sample was 12.16mg/g .The extraction time of microwave was 1/40 of supersonic extraction and extraction rate of microwave is higher than supersonic.

【关键词】 微波提取虫草素正交试验HPLC
【Key words】 microwave extractioncordycepinorthogonal test designHPLC
  • 【分类号】R284
  • 【被引频次】60
  • 【下载频次】769
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