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微波法提取虫草素研究
Study on Extraction of Cordycepin with Microwave
【摘要】 对蛹虫草固体培养物中的虫草素进行微波提取,用HPLC法测定提取液中的虫草素含量,通过正交试验,确定了微波提取虫草素的最佳条件为:提取液用量10ml,固液比1:200,中火处理3min,样品中虫草素的测定值达12.16mg/g。本法提取时间仅为超声波提取的1/40,且提取率高于超声波提取。
【Abstract】 The cordycepin in culture medium of the cordyceps militaris link were extracted by microwave treatment. HPLC was used to determine the content of cordycepin in extracting solution of cordyceps militaris link. And the orthogonal test design L9 (34) was used to optimize the conditions of microwave treatment. The optimum conditions of microwave treatment were as follows: extracting solution volume 10ml, solid-liquid ratio 1:200, middle firepower treating (microwave irradiation power 50W) for 3min. Content of the cordycepin in sample was 12.16mg/g .The extraction time of microwave was 1/40 of supersonic extraction and extraction rate of microwave is higher than supersonic.
【Key words】 microwave extraction; cordycepin; orthogonal test design; HPLC;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2006年10期
- 【分类号】R284
- 【被引频次】60
- 【下载频次】769