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固定化酶制备麦胚蛋白粉操作单元研究
Operation Unit of Wheat Germ Protein with Immobilized α-amylase
【摘要】 中空球形壳聚糖经3%戊二醛活化后与真菌α-淀粉酶在室温反应2h,冰箱静置过夜,制备固定化α-淀粉酶。固定化α-淀粉酶最适催化温度为60℃,最适pH值为5.0,相对酶活力为7.4%。固定化α-淀粉酶连续使用15d,酶活力未下降,生产麦胚蛋白质的纯度达89%,得率为79%。
【Abstract】 The hollow globular of chitosan was activated by 3% gluluraldehyde, then immobilized α-amylase for 2 hours at 25℃ and 4℃ for 12 hours. The immobilized α-amylase have the optimum reactive temperature of 60℃ at pH 5.0, and with reactive activity of enzyme of 7.4%. The immobilized α-amylase have high activity of enzyme after used 15 days. The purity of wheat germ protein is 89%, and getting ratio of 79% with immobilized α-amylase.
【关键词】 麦胚蛋白质;
固定化α-淀粉酶;
生产工艺;
【Key words】 wheat germ protein; immobilized α-amylase; producing technique;
【Key words】 wheat germ protein; immobilized α-amylase; producing technique;
【基金】 江苏省教育厅高新技术产业课题(JHB04-003)
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2006年10期
- 【分类号】Q814
- 【被引频次】3
- 【下载频次】164