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高效液相色谱法-紫外检测器测定辣椒制品中辣椒素的含量

Determination of Capsaicinoid Content in Capsicum Products with HPLC-UVD

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【作者】 王燕夏延斌熊科罗凤莲

【Author】 WANG Yan,XIA Yan-bin,XIONG Ke,LUO Feng-lian (College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)

【机构】 湖南农业大学食品科技学院湖南农业大学食品科技学院 湖南长沙410128湖南长沙410128

【摘要】 本文研究了用超声波法提取辣椒素,再采用高效液相色谱法测定。色谱条件为:色谱柱为AgilentZorbaxSB-C18(5μm,250mm×4.6mm),柱温30.0℃。UV检测器的波长:280nm。流动相V(甲醇):V(水)=80:20,流速0.8ml/min,进样量10μl。结果表明用超声波提取法简单、提取率高。

【Abstract】 This article studied the extraction of capsaicin from capsicum products by ultrasonic processing, then the capsaicin was determined by high performance liquid chromatograph(HPLC).The HPLC system consisted of Agilent Zorbax SB-C18 (5μm,250mm×4.6mm), with column temperature 30℃, and the ultraviolet detector 280nm. The mobile phase was V(Methanol): V(H2O)=80:20 with a flow rate of 0.8ml/min, and the sample volume was 10μl. The result showed that the method of ultrasonic technique is easy, the extraction rate is high, and the result is satisfactory.

【基金】 农业部948项目(2003-T18)
  • 【分类号】TS255.7
  • 【被引频次】41
  • 【下载频次】565
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