【摘要】 转基因食品的安全性得到全球的普遍关注。鉴于抗草甘膦转基因大豆导入的基因表达的5-烯醇丙酮酸莽草酸-3-磷酸合酶影响的主要是植物的次生代谢,本研究以抗草甘膦转基因大豆及其亲本和两个常规品种为材料,从大豆油、脱脂豆粉中提取酚类物质,采用高效液相色谱检测,比较了4个材料的酚类物质种类和含量。结果表明,抗草甘膦转基因大豆与其亲本和两个常规品种在多酚种类和含量上存在差异,说明采用酚类HPLC图谱法可用于鉴定不同大豆品种及其制品的来源,包括转基因大豆。这些初步研究结果有可能为转基因大豆鉴定找到一条直接、简便的方法,同时为评价转基因大豆的安全性开辟新的研究领域。更多还原

【Abstract】 The safety of genetically modified soybean(mainly Roundup Ready soybean)aroused worldwide concern and much work on its safety evaluation was carried out.Glyphosate-tolerant soybean(RR soybean)is a soybean variety that introduces an exogenous gene which controls expression of 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS).EPSPS is a key enzyme in the secondary metabolism that would affect phenolic substances metabolism.It suggests that metabolism of phenolic substances would change with respect to its parent.In this research,RR soybean and its parent,two none-genetically modified soybean varieties were used to test the phenolic substances by HPLC in the seeds of different fraction,including oil,protein and pulp.The results showed that there were significant differences in composition and content of phenolic substances between the four kinds of materials,including between RR soybean and its parent.The results suggested that the fingerprint of phenolic substances may play a role in safety evaluation of soybean variety and genetically-modified soybean.更多还原