【摘要】 采用胃蛋白酶从鱼鳞中提取胶原蛋白,考察了提取介质、前处理、搅拌、提取次数及提取时间等对胶原蛋白提取的影响。结果表明:鱼鳞胶原蛋白的最佳提取工艺为采用微波处理辅助去杂,以10%柠檬酸脱钙后,再以1%柠檬酸作为提取介质,同时加以搅拌,提取3次,每次48h。纯化后的鱼鳞胶原蛋白通过SDS-PAGE凝胶电泳和傅立叶红外光谱分析,并与标准Ⅰ型胶原蛋白比较,确定所提蛋白为典型的Ⅰ型胶原蛋白且达到了电泳纯。更多还原

【Abstract】 Collagens were extracted from fish scale of grass carp with pepsin and the effects of extraction media, pretreating, stirring, extract times and time on the yield of collagen were studied. The results indicated that the optimum extracted conditions are 1% citric acid as extraction solutions with a magnetic stirrer during the whole process, being extracted three times and 48 hours each time after removing impurity by microwave and decalcifing with 10% citric acid. Purified collagens were analyzed by SDS-PAGE and FT-IR, compared with calf tendon type Ⅰ collagen. The results confirmed that the extracted collagens are typical typeⅠ collagen and of electrophoretic purity.更多还原