【摘要】 为阐明玉米蛋白粉类胡萝卜素提取物叶黄素和玉米黄素对人口腔上皮癌细胞株KB凋亡的诱导作用,探讨其分子生物学机制。本文采用单细胞凝胶电泳、DNA凝胶电泳、流式细胞术检测叶黄素和玉米黄素诱导口腔癌细胞凋亡的情况。结果发现:单细胞凝胶电泳显示玉米蛋白粉叶黄素和玉米黄素能造成KB细胞DNA损伤、DNA凝胶电泳显示能使KB细胞DNA发生明显的降解,DNA周期分析主要阻断口腔癌细胞由G1向S期转变;流式细胞仪检测明显抑制人口腔癌细胞株KB中bcl-2基因的蛋白表达,明显升高p53、Bax基因的蛋白表达;说明玉米蛋白粉类胡萝卜素提取物叶黄素和玉米黄素具有明显抑制口腔上皮癌细胞增殖、并诱导其凋亡的作用。更多还原

【Abstract】 This study was designed to assay whether the carotenoids-lutein and zeaxanthin extracts from corn gluten meal could induce the oral cavity cancer KB cell apoptosis and illustrate the possible molecular or biology mechanism. This paper adopted single-cell gel electrophoresis (SCGE),DNA electrophoresis and flow cytometric (FCM) technique to observe the apoptosis of the cell line KB under the influence of lutein and zeaxanthin. After cells were treated with the medium containing 40 and 60μmol/L lutein or 30 and 40μmol/L zeaxanthin for 48 and 72h respectively, many cardinal apoptosis features were observed: single cell gel electrophoresis (SCGE) and DNA electrophoresis assay displayed that lutein and zeaxanthin could induce the breakage of DNA strand of KB cell; While cell cycle analysised by FCM revealed selectively the block cell cycle progression from G1 phase to S phase; and in comparison with the blank group, the expression of bcl-2 gene was low, while that of the p53 and bax genes was high. This paper suggested that the carotenoids-lutein and zeaxanthin existed corn gluten meal has the potential function of induce KB cells apoptosis.更多还原