【摘要】 本文对胡芦巴中性多糖酸水解的工艺条件进行研究。以还原糖得率为指标,采用L9(34)正交设计对酸浓度、反应时间、反应温度的参数进行优选。结果表明,从水解产物低聚糖产量考虑,6mol/L盐酸浓度,100℃反应温度,1h反应时间为最佳工艺条件,此时低聚糖的得率为43.97%,低聚糖的分子量约为500～1000Da;以获得较小分子量的低聚糖为目的,则6mol/L盐酸浓度,90℃反应温度,2h反应时间为最佳实验条件,此时低聚糖的分子量约为300～750Da,得率为13.52%。更多还原

【Abstract】 This paper studied the acid hydrolysis conditions of fenugreek neutral polysaccharide. The orthogonal test table L9 (34) was used to optimize 3 factors, namely HCl concentration, reaction time and reaction temperature. The yield of oligosaccharide was used the index in the orthogonal test. Results showed that as far as the yield of oligosaccharide was concerned, it would be as high as 43.97% when under the conditions of 6mol/L HCl, 100℃ reaction temperature and 1h reaction time, where as the molecular weight of the obtained oligosaccharide is about 500～900Da. For the final goal to obtain small molecule oligosaccharide, the optimal conditions would be 6mol/L HCl, 90℃ reaction temperature and 2h reaction time. Under the conditions, the molecular weight of the obtained oligosaccharide is about 500～1000Da and its yield is 43.97%.更多还原