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水提醇沉法从葵花籽中提取绿原酸
Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation
【摘要】 绿原酸具有抗菌、抗病毒、抗氧化等生物活性。葵花籽富含绿原酸。以水酶法提取葵花籽油脂后分离出的水浸提液为原料,采用醇沉淀法去除浸提液中的多糖,抽滤浓缩滤液后得到绿原酸。多次实验结果表明,浸提液浓缩到固形物含量在13%时,加入浸提浓缩液体积1倍量的95%的乙醇沉淀并过滤掉多糖,反复处理两次,滤液再经真空浓缩得到纯度76%左右的绿原酸。
【Abstract】 Chlorogenic acid has biological activity such as anti-microbes, anti-virus , oxidation prevention and so on. Sunflower seeds are full of chlorogenic acid. This experience extracted chlorogenic acid out of waste aqueous solution from water-enzyme method sunflower seeds oil extraction. The experiments results showed that when concentration of solid substance of the waste aqueous solution was condensated to 13% (W/V)on vacuum, 95% alcohol(1:1,V/V) was added to the condensated solution to sedimentate polysaccharose, sedimentation was centrifugalized, repeated above treatment, and filtrate was collected and condensated on vacuum, then chlorogenic acid crystal of 76% purity was obtained.
【Key words】 sunflower seeds; aqueous solution; chlorogenic acid; polysaccharose;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2006年01期
- 【分类号】TQ914.1
- 【被引频次】35
- 【下载频次】832