【摘要】 从含油的泥土中采集60份样品,通过平板检测法进行初筛,摇瓶复筛,并测定酶活。复筛菌接种于平板,由菌落直径和其水解圈直径的比筛选出诱变出发菌株,用紫外光诱变方法对菌株进行诱变,由此分离出了对富含α-亚麻酸的紫苏油具有一定专一水解能力的菌株,通过紫外诱变,其酶活较野生菌株增加了52.3%。更多还原

【Abstract】 60 samples were selected from oil dregs of oil refining plant, screened by plate and liquate medium and menstruated lipase activity, starting strain was obtained by compared diameters of colony and hydrolyzed transparency circle on screening plate. The strain were obtained by mutagenesis with UV, which has high and special hydrolyze to perilla oil with rich a-linolenic acid and its lipase activity was 52.3% higher than that original strain.更多还原