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冷冲击处理对柰李内源激素含量及蛋白质变化的影响

Effects of Cold Shock Treatments on Endogenous Phytohormones Contents and Proteins Variations of Nai-plume Fruits

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【Author】 WANG Feng1,TAN Xing-he2,GAN Lin1,WU Yong-yao3,WANG Ruo-zhong4,LI Qing-ming2 (1.College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China; 2.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3.Lab of Biochemistry and Fermentation Engineering, Hunan Agricultural University, Changsha 410128, China; 4.Hunan Provincial Key Laboratory of Phytohormones, Changsha 410218, China)

【机构】湖南农业大学园艺园林学院；湖南农业大学食品科技学院；湖南农业大学生化与发酵工程实验室；湖南省植物激素重点实验室；湖南农业大学食品科技学院湖南长沙410128；湖南长沙410128；

【摘要】本文采用了高效液相色谱(HPLC)法测定柰李果实四种内源激素(IAA,GA3,ABA,ZR)含量。通过比较了不同冷冲击处理对内源激素的影响以及贮藏过程中激素的变化,并结合冷藏过程中冷害发生情况,初步探讨了各处理在冷藏过程中内源激素变化的规律及其与柰李果实抗冷性的影响。此外,通过SDS-PAGE法分析蛋白质,结果表明,冷冲击处理能延缓柰李果实冷藏期的蛋白质降解,提高柰李果实的抗冷性。更多还原

【Abstract】 High performance liquid chromatography (HPLC) was used to detect endogenous phytohormones (IAA, GA3, ABA, ZR) of Nai-plum fruits (Prunussalicina Lindl.Var.cordata). Through comparing the variations of their contents after different cold shock treatments and the chilling injuries (CI), the regularity of phytohormone variations and the relationship between phytohormones level and the chilling tolerance of Nai-plum fruits during the cold storage were discussed. Furthermore, through SDS-PAGE analysis, the cold shock treatment was found able to delay the discomposition of proteins, hence to raise the tolerance of CI of Nai-plum fruit.更多还原

【关键词】柰李；激素；冷冲击处理；冷害；蛋白质；贮藏；
【Key words】 Prunussalicina Lindl.Var.cordata； phytohormones； cold shock treatment； chilling injury； protein； storage；

【基金】湖南省自然科学基金(04JJ6046)

【文献出处】食品科学 ,Food Science , 编辑部邮箱 ,2006年01期

【分类号】S662.3
【被引频次】17
【下载频次】259

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