【摘要】 通过单因素和正交试验,对菌核侧耳产β-葡萄糖苷酶液体培养条件进行优化。结果表明:以黄豆浆为氮源,葡萄糖为碳源,初始pH为6.0产酶条件最优。培养温度为25℃、装液量50ml/250ml、摇瓶转速为150r/min培养72h后,酶活力达最高,为1.86U/g(湿菌丝体)。更多还原

【Abstract】 The optimum liquid culture conditions producing β-glucosidase from Pleurotus tuber regium (Fr.) Sing were studied. The optimum conditions for fermentation were: the soybean milk as the nitrogen source, the glucose as the carbon source, the initial optimum pH was 6.0. Under the optimal incubation condition: culture temperature was 25℃, volume 50ml/250ml, rotate speed 150r/min and culture time 72h, the highest β-glucosidase activity was obtained at wet weight of 1.86U/g (wet mycelium).更多还原