【摘要】 采用摇瓶发酵法对灰树花液态发酵的碳氮营养需求及发酵条件对灰树花茵丝体的生物量与多糖产量规律进行了研究。结果表明,灰树花具有较广泛的碳源谱和氮源谱,最佳发酵培养基是葡萄糖-麸皮-豆饼粉培养基。最适发酵条件为25℃,摇床转速100r/min,接种量10％,装液量为250mL三角瓶装100mL发酵液,pH在5．0～6．0,发酵周期为8d,在上述营养和发酵条件下菌丝体生物量积累最多,同时灰树花多糖可达到较高的产量。更多还原

【Abstract】 The appropriate liquid fermentation medium and submerged fermentation condition for Grifola frondosa,and the regulation of the production of biomass and crude polysaccharides by Grifola frondosa were studied.The results showed: various carbon and nitrogen sources could be used,but the best liquid fermentation medium was made up of glucose,bran,and skin-bean cake.The optimal composition of the medium was obtained and the optimum culture conditions by shaking flask culture were:100 mL medium in 250 mL flask,pH5～6,10% inoculating amount, shaking at temperature of 25℃100 r/min for 8days.更多还原