【摘要】 选用中性蛋白酶和胃蛋白酶水解鸡脑蛋白,确定最佳水解条件为:先采用中性蛋白酶于50℃,pH7.0,加酶量0.8U/mg蛋白质,底物浓度0.5%,水解4h;再用胃蛋白酶于40℃,pH1.0,加酶量8U/mg蛋白质,水解3h。在该条件下,肽得率为70.38%,水解产物的氨基酸含量为2.01%,10kDa分子量以下的肽含量为47.72%,3kDa分子量以下的肽含量为40.14%,1kDa分子量以下的肽含量为29.03%。更多还原

【Abstract】 Chicken’s cerebroprotein is enzymatically hydrolysated using neutral protease and pepsin. The optimum condition for the hydrolysis by the two enzymes is hydrolysis with neutral protease at 0.8U/mg protein,pH7.0 and 50℃ for 4 hours,the substrate’s concentration was 0.5% and then with pepsin at 8U/mg protein,40℃ and pH1.0 for 3 hours. The peptide,amino acid,peptide of molecular weight below 10kDa,3kDa,1kDa are 70.38%,2.01%,47.72%,40.14%,29.03% respectively.更多还原