This study was aimed to purify hypocholesterolemic peptides from soy protein isolate. Hydrolysate of soy protein isolate which was hydrolyzed by Alcalase was desalted through macroporous adsorption resin, then eluted by ethanol with different concentration according to its hydrophobic property. Then the most hypocholesterolemic activity fraction which was eluted by ethanol with 75% concentration was fractionated with Sephadex G-15 gel filtration and the most active peptides in which MW is below 1 000, with ...