【摘要】 建立了间接ELISA检测盐酸克伦特罗的方法,并对其参数进行了分析计算。在该检测方法中,抗盐酸克伦特罗(CL)抗体最适稀释度为1∶1000,羊抗兔酶联抗体(HRP-IgG)的最适稀释度为1∶1500。该检测方法的检测灵敏度可达0·1046μg/L,生物检测限为1·452μg/L,线性检测范围为7·26~90·75μg/L。更多还原

【Abstract】 An indirect enzyme-linked immunosorbent assay(ELISA) was developed to detect and quantitate clenbuterol. The rabbit antiserum containing polyclonal antibodies against clenbuterol was prepared successfully by using BSA-CL as antigen. The antibody was purified with ammonium sulfate fractionation. The sensitivity of the assay is 0.104 6 μg/L and the biologic limit of detection was 1.452 μg/L, the standard curve was linear from 7.26 to 90.75 μg/L.更多还原