【摘要】 以纳豆芽孢杆菌(Bacillus natto)S004为出发菌,经诱变选育后得到S004-50-01菌株,聚谷氨酸产量由原菌株的4.25 g/L提高到10.0 g/L,对谷氨酸的利用率由18.2%提高到42.83%。通过正交实验,获得该菌株的优化培养条件为:葡萄糖40 g/L,谷氨酸钠30 g/L,MgSO40.5 g/L,FeCl30.5 g/L,MnSO40.02 g/L,K2HPO43.0 g/L,经72 h发酵培养,聚谷氨酸的平均产量为13.6 g/L,谷氨酸的利用率为45.33%。更多还原

【Abstract】 In the present study,Bacillus natto S004-50-01 was screened from its parent strain of Bacillus natto S004 by ultraviolet radiation.γ-Polyglutamic acid yield of the strain increased from original 4.25 g/L to 10.0 g/L,the utilization ratio of glutamic acid enhanced from original 18.2% to 42.83%.The optimal result was obtained that the culture medium was glucose 40 g/L,sodium glutamate 30 g/L,MgSO₄ 0.5 g/L,FeCL₃ 0.5 g/L,MnSO₄ 0.02 g/L,and K₂HPO₄ 3.0 g/L by orthogonal experiment.After fermented cultivation in 72 hours,the average yield of γ-Polyglutamic acid was 13.6 g/L,the utilization ratio of glutamic acid was 45.33%.更多还原