【摘要】 利用 DA201-C 大孔吸附树脂对螺蛳腹足肌酶解产物进行脱盐及糖原分离。结果表明,用不同浓度的乙醇进行洗脱时,洗脱组分的疏水性氨基酸含量及 ACE 抑制活性随乙醇浓度的增大而增大；依次用25%和 75%乙醇进行阶段洗脱时,脱糖率为78．59%,脱盐率为93．79%；酶解糖原比碱提糖原的相对分子质量大而纯度较低。更多还原

【Abstract】 DA201-C macroporous adsorption resin was used to purificate Bellamya purificata Hydrolysates. The result showed that when different concentrations of alcohol 25%,50% and 75% were used to flush the medi- um,the hydrophobic amino acid and the ACE inhibiteing rate of the fraction increased with the alcohol concentra- tion.When 25% and 75% alcohol was used to elute the medium,the desalting ratio of 93.79% and 78.59% glycogen was obtained.The Mw of glycogen extracted by enzymatic method was higher and the purity was lower than that extracted by alkaline method.更多还原