【摘要】 目的探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)的关系。方法采用聚合酶链反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病病人的ApoH外显子3、8基因型。结果冠心病组外显子3的GG基因型频率为81.8%,GA+AA基因型频率为18.2%,G等位基因频率为88.0%,A等位基因频率为12.0%,与对照组比较差异无显著性(χ2=2.147、1.454,P>0.05)。冠心病组外显子8的GG基因型频率为74.5%,GC基因型频率为25.5%,G等位基因频率为87.0%,C等位基因频率为13.0%,与对照组比较,冠心病组的GC基因型及C等位基因频率显著增高(χ2=7.742、3.907,P<0.05)。结论ApoH外显子3基因多态性与冠心病无相关性;ApoH外显子8 GC基因型及C等位基因与冠心病有关。更多还原

【Abstract】 Objective To investigate the relationship between polymorphism of apolipoprotein H(ApoH) exon 3 and(exon 8) with coronary heart disease(CHD).MethodsPolymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of ApoH exon 3 and exon 8 in 110 patients with CHD and 100 healthy adults. Results The frequency of genotype GG of exon 3 in the CHD group was 81.8%;in GA+AA genotype,18.2%;in G allele,(88.0%);in A allele,12.0%.There was no significant difference compared with the controls(χ~2=2.147,1.454;P>0.05).The frequency of GG genotype of exon 8 was 74.5%;GC genotype,25.5%;G allele,87.0%.GC genotype and C allele in CHD group were significantly higher than that in the control group(χ~2=7.742,3.907;P<0.05).ConclusionThe polymorphism of the apolipoprotein H exon 3 has no relationship with CHD,but the GC genotype of ApoH exon 8 and C allele have close relation with CHD.更多还原