Molecular beacon is a kind of high sensitive and selective fluorescence oligonucleotide probes that is producing fluorescence upon hybridizing with their complementary DNA or RNA targets.Based on the genome sequence of the ayr subtype published on Genebank,total 6 pieces of molecular beacon probes were obtained through Beacon Designer 4.0 software in order to offer some referencs and clues for rapid HBV virus diagnosis in clinical applification.