【摘要】 细胞质膜是构成细胞对外界环境的屏障和细胞内外环境交流的界面,镶嵌或连接于其中的蛋白质参与细胞/细胞以及细胞/细胞外基质的识别、信号的接受和跨膜传导、细胞内外物质的转运;此外,质膜蛋白质在药物研发中也起着非常重要的作用,在现有的药靶中质膜蛋白质占70%.因此质膜蛋白质组学研究成为亚细胞蛋白质组学研究的热点.然而在质膜蛋白质组学的研究中,由于很难获得高纯度的质膜样品,因此这一领域的研究具有很大的挑战性.现主要对质膜及其微区结构的纯化方法和质膜纯度的评价标准作扼要的介绍.更多还原

【Abstract】 Plasma membrane(PM) is an organized system serving as a structural and communication interface with the extracellular environment for exchanges of information and substances.Consequently the functions of proteins embedded in this membrane include cell/cell and cell/extracellular matrix recognition,the reception and transduction of extracellular signals and the transport of solutes and water molecules into and out of the cell.Moreover,the plasma membrane proteins account for 70%of all known drug targets.For these reasons,proteomic analysis of plasma membrane is one of the focuses of subcellular proteome research.However,the plasma membrane proteomics is much challenged mainly due to the difficulty to obtain the purified plasma membrane.The progress of plasma membrane proteomics including the purification of plasma membrane and its microdomains,and the purification evaluation methods are summarized.更多还原