【摘要】 采用差速贴壁法体外原代培养大鼠心肌细胞;NPY刺激培养的心肌细胞增殖;RNA干涉特异性抑制CaN的活性,阻断NPY刺激的心肌细胞中Ca2+/CaM-CaN信号转导通路;观察对CaN活性、表达水平和心肌细胞蛋白合成速率的变化.实验结果显示NPY可增加心肌细胞的CaN活性和表达,加快细胞内蛋白合成速率.RNA干涉抑制CaN活性后,明显降低NPY刺激的蛋白合成速率.CaN参与了NPY刺激的心肌细胞增殖,RNA干涉通过抑制CaN的活性可阻断N PY诱导的心肌细胞肥大Ca2+/CaM-CaN通路.更多还原

【Abstract】 To investigate the effects of Ca2+ /CaM-dependent calcineurin(CaN) signaling pathway on cardiomyocytes hypertrophy of rat induced by neuropeptide Y(NPY),and the inhibition of siRNA on the activity of CaN and synthesis of cardiomyocytes protein.Cardiomyocytes of neonatal Wistar rats were cultured with 100 nmol/L NPY,RNA interference(RNAi) was used to block CaN-depended signal transduction pathway in rat cardiomyocytes stimulated by NPY,then the activity and the expression level of CaN,proliferation of cardiomyocytes were observed.Results showed that NPY could increase CaN activity and the proliferation of cardiomyocytes.While RNAi inhibited the activity of CaN and cardiomyocytes proliferation.Neumpeptide Y activates Ca2+ /CaM-dependent calcineurin signal pathway,while the activity of CaN and cardiomyocytes proliferation stimulated by NPY could be blocked by RNAi.更多还原