【摘要】 近几十年来,亲和层析技术发展十分迅速,广泛应用于生物分子(如结合蛋白、酶、抑制剂、抗原、抗体、激素、激素受体、糖蛋白、核酸及多糖类等)及组织(如细胞、细胞器、病毒等)的分离和纯化,是蛋白质组学研究中重要的技术之一.介绍了亲和层析的基本类型及配体合成的研究进展,概述了亲和层析技术在蛋白质组学以及在其他方面的应用和发展动态.更多还原

【Abstract】 The development of affinity chromatography technique is very fast in recent years,remarkable ac-complishments have been achieved in the separation and purification of biomolecules and organisms as well as in studying the interactions between biomolecules.The commonly used different ligand types and recent progress in searching for ligands are demonstrated.The application of affinity chromatography in proteomics and in other areas are outlined.更多还原