【摘要】 目的:探讨枸杞多糖(LBP)对人肺癌A549细胞的影响及作用机制。方法:将LBP作用于肺癌A549细胞,应用M TT法、电镜和钙离子测定等技术测定LBP的抗肿瘤作用及作用机理。结果:LBP呈剂量依赖性(100m g/L~2000m g/L)地抑制人肺癌A549细胞的增殖,诱导其凋亡,并可使细胞内钙离子浓度升高。结论:LBP可诱导A549细胞凋亡,其作用机制可能与细胞内钙离子浓度升高有关。更多还原

【Abstract】 Objective: To investigate the effect and mechanism of LBP on Apotosis of Human Lung Cancer Cells.Methods: Cells was incubated with LBP.The effect was detected by MTT,electronic microscope and level of intracellular free calcium concentration.Results: LBP inhibited the growth of A₅₄₉ cells in dose-dependent manner and induced the apoptosis of them.And the level of intracellular free calcium concentration in the A₅₄₉ cells was significantly higher than that of the control group.Conclusion: LBP can induce the apoptosis of A₅₄₉ cells,and one of its molecular mechanism may be associated with the rising of the level of intracellular free calcium concentration.更多还原