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青枯菌侵染桉树幼根的组织病理学研究
Histopathological study on young roots of eucalyptus invaded by Ralstonia solanacearum
【摘要】 接种青枯菌后不同时间切取桉树幼根根表、根横切面和纵切面进行制样,扫描电镜观察其组织病理学变化。结果显示:接种0.5 h后即有少量病菌存在于根表细胞间隙和导管间隙,但寄主表皮细胞和维管束结构基本完好;接种2 h,表皮细胞壁被破坏,细胞瓦解;接种24 h,表皮细胞间出现较大裂隙.在横切面和纵切面上可观察到大量细菌聚集并结为白色絮状物.导管破裂并被堵塞;此时植株外观上也开始出现萎蔫症状。
【Abstract】 The epidermis,cross and vertical sections of young roots of a eucalyptus clone inoculated with Ralstonia solanacearum for various time were excised and prepared for scanning electron microscopy(SEM)observation.Only a few bacteria were present in the intercellular spaces of epidermal tissue and the spaces between vessels 0.5 h after the inoculation,the epidermal ceils and vascular tissue,however,were virtually unaffected.The cell walls of epidermis were broken and the cells were disintegrated 2 h after the inoculation.Big cracks appeared on the epidermis,massive bacteria aggregated in white flocs,the vessels were blocked and disrupted 24 h after the inoculation when while the wilt symptom began to show on the eucalyptus ramet.
【Key words】 Ralstonia solanacearum; eucalyptus; infection; bistopathology; SEM;
- 【文献出处】 中国森林病虫 ,Forest Pest and Disease , 编辑部邮箱 ,2006年06期
- 【分类号】S763.3
- 【被引频次】3
- 【下载频次】207