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青枯菌侵染桉树幼根的组织病理学研究

Histopathological study on young roots of eucalyptus invaded by Ralstonia solanacearum

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【作者】 王军孙思韦爱梅

【Author】 WANG Jun,et al.(College of Forestry,South China Agricultural University,Guangzhou 510642,China)

【机构】 华南农业大学林学院东莞市林业局 广东广州516042广东广州516042广东东莞511700

【摘要】 接种青枯菌后不同时间切取桉树幼根根表、根横切面和纵切面进行制样,扫描电镜观察其组织病理学变化。结果显示:接种0.5 h后即有少量病菌存在于根表细胞间隙和导管间隙,但寄主表皮细胞和维管束结构基本完好;接种2 h,表皮细胞壁被破坏,细胞瓦解;接种24 h,表皮细胞间出现较大裂隙.在横切面和纵切面上可观察到大量细菌聚集并结为白色絮状物.导管破裂并被堵塞;此时植株外观上也开始出现萎蔫症状。

【Abstract】 The epidermis,cross and vertical sections of young roots of a eucalyptus clone inoculated with Ralstonia solanacearum for various time were excised and prepared for scanning electron microscopy(SEM)observation.Only a few bacteria were present in the intercellular spaces of epidermal tissue and the spaces between vessels 0.5 h after the inoculation,the epidermal ceils and vascular tissue,however,were virtually unaffected.The cell walls of epidermis were broken and the cells were disintegrated 2 h after the inoculation.Big cracks appeared on the epidermis,massive bacteria aggregated in white flocs,the vessels were blocked and disrupted 24 h after the inoculation when while the wilt symptom began to show on the eucalyptus ramet.

【关键词】 青枯菌桉树侵染组织病理学扫描电镜
【Key words】 Ralstonia solanacearumeucalyptusinfectionbistopathologySEM
【基金】 国家自然科学基金(30471395)资助项目
  • 【文献出处】 中国森林病虫 ,Forest Pest and Disease , 编辑部邮箱 ,2006年06期
  • 【分类号】S763.3
  • 【被引频次】3
  • 【下载频次】207
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