【摘要】 目的观察诱生型血红素氧合酶(HO-1)mRNA、诱生型一氧化氮合酶(iNOS)mRNA在局灶性脑缺血中的表达及其不同作用。方法采用逆转录酶多聚酶链反应(RT-PCR)方法,测定HO-1mRNA、iNOSmRNA在局灶性缺血脑组织中不同时间点的表达变化。结果iNOSmRNA的表达在缺血后2 h出现,24 h达最高峰,以后逐渐下降。HO-1mRNA表达在缺血后2 h即出现,缺血后12 h达最高峰。结论脑缺血的病理生理过程中存在着一氧化氮(NO)及一氧化碳(CO)两种信使系统之间的相互作用。HO-1mRNA及iNOSmRNA的表达上调并具有时相性。缺血后期HO-1mRNA仍然维持在一定的水平,可能具有对抗后期iNOSmRNA增高所产生的NO毒性作用。更多还原

【Abstract】 Objective To study the simultaneous expression of heme oxygenase(HO)-1 mRNA and(inducible) nitric oxide synthase(iNOS) mRNA during permanent focal cerebral ischemia in rats.Methods The(expressions) of HO-1 mRNA and iNOS mRNA in brains of 48 rats during permanent focal cerebral ischemia were(investigated) by means of reverse transcriptase polymerase chain reaction(RT-PCR) on the basis of model of(middle) cerebral artery occlusion(MCAO).Results iNOS mRNA was detected in ischemic cerebral tissue at 2 h after ischemia,peaked at 24 h,then slowed down,till 7 d after MCAO.HO-1 mRNA can be detected at 2 h after MCAO.The intensity of HO-1 mRNA increased with time,peaked at 12 h,then slowed down till 7 d after MCAO.Conclusion The expressions of HO-1 mRNA and iNOS mRNA both up-regulated and have characteristic of time course.In late stage of ischemia,the expression of HO-1 mRNA to some extent protects against increasing toxicity of nitric oxide(NO) incurred by increasing iNOS mRNA.更多还原