【摘要】 目的研究不同声压级次声作用小鼠后海马内白细胞介素-6(interleuk in,IL-6)mRNA的改变和意义。方法BALB/C小鼠暴露于16 Hz、声压90 dB和130 dB次声。每天作用2 h,分别作用1、7、14、21和28 d后,采用原位杂交方法观察小鼠海马内IL-6 mRNA的改变。结果发现90 dB和130 dB次声作用后IL-6 mRNA的表达在海马区域明显增多;130 dB次声作用较90 dB次声作用强;在相同强度的次声作用下,作用次数的多少与海马内IL-6 mRNA的改变程度呈正相关。结论海马区域对次声敏感,次声的作用效应与声压级和作用时间有关;次声可以通过海马内IL-6 mRNA的改变造成脑损伤,这是次声导致脑损害的重要因素之一。更多还原

【Abstract】 Objective To study the change of interleukin-6(IL-6) mRNA in the hippocampus of mice exposed to infrasound of different sound pressure.Methods The BALB/C mice were exposed to infrasound of 16 Hz with(intensity) of 90 dB and 130 dB for 1,7,14,21 and 28 d,2 h per day.Then in situ hybridization technique was(employed) to detect the change of IL-6 mRNA in the brains of the mice.Results The expression of IL-6 mRNA(increased) in the hippocampus regions after exposed to infrasound of 90 dB and 130 dB.The effect of 130 dB(infrasound) was large than that of 90 dB.Conclusion The extent of IL-6 mRNA change is closely related to the action time under infrasound of the same sound pressure level.Infrasound brings about brain injury through the change of IL-6 mRNA in the hippocampus,which is one of the important factors,by which the infrasound cause brain injury.更多还原