【摘要】 研究了快速测定葡萄酒中有机酸的高效液相色谱(HPLC)法,其最佳条件是:色谱柱Aminex HPX-87H柱(300×7.8mm),流速0.6mL/min,进样体积10μL,检测波长210nm,柱温55℃,流动相0.005 mol/L硫酸溶液与去离子水配比为90∶10。该方法的保留时间变异系数在0.037%~2.144%,有机酸变异系数在0.5305%~2.1559%,有机酸回收率大都在85.95%~101.66%,精确度高、重现性好。更多还原

【Abstract】 The high performance liquid chromatogram(HPLC) method for the determination of organic acids in wine was studied.The established HPLC optimal conditions were followed:Aminex HPX-87H column(300×7.8mm) was used,the HPLC chromatographic separation was carried out by using 0.005mol/L sulfuric acid in ultra pure water with the ratio of 90:10,the flow-rate of the eluent was 0.6 mL/min and the column temperature was 55℃,for whole-wavelength monitoring,the detector was set at 210 nm for all acids.The established HPLC method,with reproducibility was better than 0.037%～2.144% for retention time,precision better than 0.5305%～2.1559% for organic acids and recoveries of organic acids mostly in the 85.95%～101.66% range,was suitable well for the determination of organic acids in wine.更多还原