【摘要】 利用mRNA差异显示技术,对入孵67~91h的鸡(♀)与鹌鹑(♂)杂交种胚胎中mRNA差异表达进行比较,筛选出与杂交种胚胎早期发育相关的特异表达基因,并克隆测序这些序列,分析这些基因在杂交受精蛋早期胚胎发育中的作用,及其对杂交种早期胚胎死亡是否有影响。分析结果表明,已经获得6个杂交种孵化不同时期的基因片段与鸡的mRNA序列具有高度同源性。其中,孵化第72h时胚胎mRNA表达的序列与鸡雌激素受体结合位点相关抗原mRNA具有高度同源性,孵化第79h时产生的差异片段经过比对,发现:与鸡(♀)肽酰-脯氨酰异构酶类似的mRNA序列具有高度同源性。而杂交种胚胎发育到第72h时,可检测到鸡雌激素受体结合位点相关抗原mRNA的表达,比前人所得到的表达时间(胚胎发育在第84h时检测到)提前了12h,这很可能是造成杂交种早期胚胎大量死亡的原因之一。更多还原

【Abstract】 We applied mRNA Differential Display to screen different genes expression during early embryonic stage of chicken and quail hybridization,we cloned the differential gene segments and analysed these sequences’ relationship with the embryonic development of the early stage hybridization,and presumed the main infection to the death of the early embryo.The result shows that six genes have high homology with several chicken mRNA.One gene segment of 364bp contrasted with Gene bank,observed that it is highly conformability with estrogen receptor binding site associated antigen mRNA.And the other 229bp gene is similar to peptidyl prolyl isomerase enzyme mRNA.We found the estrogen receptor binding site associated antigen mRNA may have main infection with the early embryonic development.更多还原