【摘要】 为研究Neuritin蛋白的功能,将neuritinORF在杆状病毒-昆虫表达系统中表达,构建杆状病毒表达载体。以308D10为模板,利用PCR方法扩增neuritinORF,定向克隆法将其克隆至PFASTBAC-HTA转移载体中。然后利用同源重组原理将其转移至杆粒bacmid中。得到携带neuritin ORF的重组杆粒。本实验获得了重组目的基因真核表达载体并经PCR鉴定,插入片段方向、大小正确,DNA测序分析表明插入处接头和读框与预期序列相符,成功构建了Neuritin的杆状病毒表达载体。更多还原

【Abstract】 To research the function of Neuritin.Constructed an expression vector carrying neuritin gene in baclovirus-insect system was constructed.The open reading frame of neuritin was cloned into the vector PFASTBAC-HTA.Then we recombined the gene into bacmid.The structure of the baclovirus vectors carrying neuritin gene was identified by PCR analysis conclusion the neuritin gene was recombined into the bacmid and the baclovirus expression vector of Neuritin was constructed successfullly.更多还原