【摘要】 目的研究急性特发性血小板减少性紫癜(ITP)患儿外周血T细胞蛋白激酶C(PKC)的活性变化及其与T细胞活化和血小板减少程度之间的关系。方法无菌采集40例急性ITP患儿及35名健康儿童外周血,T细胞分离富集柱法分离纯化T细胞,用非同位素标记法检测T细胞PKC的活性变化,酶联免疫吸附试验(ELISA)检测血清可溶性白细胞介素-2受体(sIL-2R)水平,血液分析仪计数血小板。结果ITP患儿T细胞PKC的活性为(0.98±0.22)nmol.m in-1.m l-1,与正常健康儿童[(0.53±0.13)nmol.m in-1.m l-1]相比明显增强(P<0.05),T细胞活化标志sIL-2R的表达[(528±124)U/m l]与正常儿童[(296±57)U/m l]比较显著升高(P<0.05),T细胞PKC的活性变化与sIL-2R的表达呈显著正相关(r=0.872 50,P<0.05),与血小板计数呈显著负相关(r=-0.810 67,P<0.05)。结论急性ITP患儿外周血PKC活性增强可能导致T细胞活化,活性T细胞增多可能引起患儿血小板的损伤,提示PKC信号传导在ITP的免疫病理机制中发挥重要作用。更多还原

【Abstract】 Objective To investigate the change of protein kinase C（PKC） activity in peripheral blood T lymphocytes in acute idiopathic thrombocytopenic purpura（ITP） children and the relationship between PKC activity,T lymphocytes activation and thrombocytes decrease. Methods Collecting sterile peripheral blood from acute ITP children（n=40） and healthy children（n=35）,T lymphocytes were isolated and purified by the T cell segregation enrichment column.The PKC activity was detected by non-radioactive assay,sIL-2R,which was T cell activated marker,was determined by enzyme-linked immunoabsorbent assay（ELISA）.Platelet was counted by cell counting meter. Results Compared with healthy children,PKC activity was significantly enhanced in acute ITP children [（0.98±0.22） nmol·min^-1·ml^-1 vs（0.53±0.13） nmol·min^-1·ml^-1,P<0.05].Expression of sIL-2R in serum was significantly improved [（528±124） U/ml vs（296±57） U/ml,P<0.05].Besides,relationship between the changes of PKC activity and sIL-2R was positive correlation（r=0.872 50,P<0.05）,however,relationship between PKC activity and platelet count was significantly negative correlation（r=-0.810 67,P<0.05）. Conclusions Enhancement of PKC activity in acute ITP children may relate to T lymphocytes activation and result in reduction of thrombocytes,which indicates that PKC signal transduction way may play an important role in the immunopathogenesis of acute ITP.更多还原