【摘要】 目的采用聚合酶链反应(PCR)-寡核苷酸微流芯片进行人乳头状瘤病毒(HPV)分型,并研究子宫颈上皮内瘤变(CIN)与HPV感染型别的关系。方法对335例临床病例进行筛查、细胞学分级并分组,活检标本通过寡核苷酸微流芯片上特异性探针进行HPV分型。结果335例患者中,HPV阳性率为22．1%。宫颈良性疾病组的HPV阳性率为10．9%,CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌组分别为47．2%、66．7%、68．4%和75．0%,均显著高于宫颈良性疾病组(P值均＜0．05)。结论随着CIN级别的升高,高危HPV型感染比例增加,寡核苷酸微流芯片检测能区分多种HPV型别,可用于临床宫颈上皮内瘤变的HPV筛查。更多还原

【Abstract】 Objective To study the relationship between intraepithelial cervical neoplasia(CIN)and types of Human papilloma virus(HPV)infection.Methods 335 cases were collected and grouped by cervical cytologic analysis.The HPV of CIN lesions was typed by microfluid chip.Results Of the 335 patients,the total HPV posi- tive rate was 22.1%(74/335).The prevalence of benign cervical squamous epithelium group was 10.9%(28/ 256).Out of 36 CIN I lesions,17 were HPV DNA positive(54%).In CINⅡgroup,66.7%(10/15)was associ- ated with HPV infection.68.4%(13/19) of CINⅢlesions were positive for HPV whereas 75%(6/8)of cervical carcinoma showed HPV positive.They showed significant increases in prevalence as comparing with cervical aqua- mous epithelium group(P＜0.05).Conclusion The percentage of HPV infection increases with increase of CIN grade,demonstrating that the Oligo DNA microfluid chip assay can discriminate many types of HPV and can be ap- plied for HPV screening of clinical CIN.更多还原