【摘要】 目的研究他莫昔芬对结肠癌细胞株LoVo的促凋亡作用及其机制。方法采用流式细胞仪测定他莫昔芬对结肠癌细胞株LoVo的促凋亡作用。采用免疫印迹法测定雌激素受体(ER)在结肠癌细胞株LoVo中的表达及他莫昔芬对生存素表达的影响。结果流式细胞仪检测显示,1×10-6、1×10-5、1×10-4mol/L浓度的他莫昔芬作用于LoVo 48 h后的凋亡率分别为59．24％、17．01％和7．33％。1×10-4和1×10-5mol/L他莫昔芬组的凋亡率与对照组的显著有差异性(P<0．01)。结肠癌细胞株LoVo中,ERβ有高表达,而ERα没有表达。进一步研究发现,他莫昔芬引起LoVo细胞生存素表达降低,而5-氟尿嘧啶(5-FU)对生存素表达没有影响。结论他莫昔芬有显著诱导LoVo细胞凋亡的作用,其促凋亡机制可能是以ERβ为靶点使人结肠癌细胞株LoVo的生存素表达下降。更多还原

【Abstract】 Objective To evaluate apoptosis of LoVo colon cancer cells induced by tamoxifen and to investigate its mechanism. Methods Apoptosis of the cells was measured by TUNEL method and flow cytometry. The expression of ERs and survivin were determined by Western blot method. Results Treatment with TAM at a concentration of 1×10-5 and 1×10-4 mol/L, the apoptosis increased at a apoptotic percentage of 17. 01% and 59. 24% , respectively by flow cytometer. The apotosis rate between TAM and controlled group showed significant defference at 1×10-4 and 1×10-3 mol/L. The expression of ERβ could be detected in LoVo cells, while the expression of ERα could not. Further study showed that TAM can decrease the expression of survivin. 5-FU while can’t do so. Conclusions TAM induces LoVo cells apoptosis in vitro with the mechanism of inhibiting survivin expression.更多还原