【摘要】 目的制备适合分离纯化磷酸激酶的亲和色谱介质。方法以纸纤维为载体,环氧氯丙烷等为活化剂,考察接臂、配基对磷酸激酶吸附效果的影响,确定亲和色谱介质制备的工艺路线,并对主要工艺参数进行优化。结果己糖激酶纯化倍数可达20倍以上,酶活回收率近60%。结论以自制的亲和色谱介质分离纯化啤酒酵母中的磷酸激酶,成本低,操作简单,分离效果良好。更多还原

【Abstract】 PurposeTo synthesize the affinity chromatography medium to purify kinase.MethodsThe supporter paper fiber was activated with epoxy chloropropane etc.According to the influence of spacer and different ligand on absorption of kinase,process route was optimized.ResultsMore than 20-fold purified Hexokinase was obtained in one step with a yield of 60%.ConclusionThis method is suitable for to kinase purification owing to its low cost and simple operation.更多还原