【摘要】 目的探索一种新型的免疫调节剂,充分利用动物胎盘。方法利用超滤法从牛和羊胎盘中提取胎盘肽,采用改良的加脲Tricine-SDS-PAGE法测定其相对分子质量;利用紫外光扫描其最大吸收峰;并通过建立体外抑制兔淋巴细胞模型,利用四甲基偶氮唑盐(MTT)比色法测定所提取胎盘肽的免疫调节作用。结果从牛和羊胎盘中成功提取出了胎盘肽,其相对分子质量分别为4 785和4 386;其最大吸收峰分别为210.5和225.4 nm;牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高,其中牛胎盘肽以1∶1×103、1∶1×1042组吸收度明显高于抑制组(P<0.05);羊胎盘肽以1∶1×102、1∶1×103和1∶1×1043组效果最为明显(P<0.01)。结论牛和羊胎盘中含有的胎盘肽可成功提取出来,其可明显提高淋巴细胞的转化率,是一种较为理想的免疫调节剂。更多还原

【Abstract】 Purpose To search for a new immunoregulator and to utilize animal placenta adequately.MethodsThe distilled bovine and caprine placental peptides were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.Their absorbance peaks were got by ultraviolet scanning.Their immnoregulatory effects were mensurated by MTT colorimetric assay after establishing a model of in vitro immunosuppressive lymphocytes of rabbit.ResultsThe distilled bovine and caprine placental peptides were prepared successfully.Their relative molecular weights were 4 785 and 4 386 respectively.And their absorbance peaks were 210.5 nm and 225.4 nm respectively.The bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate.The immunosuppressive lymphocyte transformation rates in groups with the bovine placental peptide of 1∶1×10~3 and 1∶1×10~4(P<(0.05))and with the caprine placental peptides of 1∶1×10~2,1∶1×10~3and 1∶1×10~4(P<0.01)increased more remarkably than that in immunosuppression group.ConclusionThere were placental peptides in bovine and caprine placenta which could be distilled successfully.And they could improve immunosuppressive T lymphocyte transformation rate remarkably,and may be a reasonable immunoregulator.更多还原